Note citations are based on reference standards however formatting rules can vary widely between applications and fields of interest or study the specific requirements or preferences of your reviewing publisher classroom teacher institution or organization should be applied. Journal of chromatography a 1119 2006 140 146 use of ultra performance liquid chromatography in pharmaceutical development stephen ac wren pierre tchelitcheff analytical development pard astrazeneca silk business park macclesfield cheshire sk10 2na uk. High performance liquid chromatography hplc formerly referred to as high pressure liquid chromatography is a technique in analytical chemistry used to separate identify and quantify each component in a mixture it relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Liquid chromatography mass spectrometry lc ms is an analytical chemistry technique that combines the physical separation capabilities of liquid chromatography or hplc with the mass analysis capabilities of mass spectrometry ms coupled chromatography ms systems are popular in chemical analysis because the individual capabilities of each technique are enhanced synergistically. Ultra performance liquid chromatography uplc has been investigated as an alternative to hplc for the analysis of pharmaceutical development compounds we present data on three compounds showing that significant reductions in separation time can be achieved without compromising the separation quality
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